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Ch AT-mh Ch R2-YFP mice derived from founder line 6 exhibit strong EYFP expression in striatum, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei.Ch AT co-staining shows precise co-localization with mh Ch R2-EYFP expression neurons; suggesting mh Ch R2-EYFP labeled neurons in this line are cholinergic neurons.The mh Ch R2:: YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mh Ch R2; also called h Ch R2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP).The mh Ch R2 is designed to cause larger stationary photocurrents compared to Ch R2.The donating investigator also reports that these Ch AT-mh Ch R2-YFP line 6 mice exhibit a similar expression pattern as Ch AT-mh Ch R2-YFP line 5 (Stock No. The BAC also includes the vesicular acetylcholine transporter gene (Slc18a3 or VACh T) that, because it is located in the intron between the first and second exons of the Chat gene, is under the control of the Chat promoter.014545), but line 6 mice have brighter EYFP expression. The resulting ~220 kb Ch AT-mh Ch R2-YFP BAC transgene was injected into B6SJLF1 fertilized oocytes.Ch AT co-staining shows precise co-localization with mh Ch R2-EYFP expression neurons; suggesting mh Ch R2-EYFP labeled neurons in this line are cholinergic neurons. This mh Ch R2:: YFP fusion protein (also called h Ch R2-H134R-EYFP) sequence was inserted into the coding region of the mouse choline acetyltransferase (Chat or Ch AT) locus on the RP23-246B12 bacterial artificial chromosome (BAC) via homologous recombination.High EYFP fluorescence is also observed in the ventral gray horn in transverse section of the spinal cord. This was designed to disable transcription from the Chat locus, and none of the other loci on the BAC were altered.
We will fulfill your order by providing at least two carriers for each strain ordered.The BAC used to generate the construct includes the vesicular acetylcholine transporter gene (Slc18a3 or VACh T).Slc18a3 is located in the intron between the first and second exons of the Chat gene and is under the control of the Chat promoter.The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mh Ch R2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials.As such, illuminating mh Ch R2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.